Monday, 3 October 2016

LaCONES: CSIR Takes Steps for Nurturing Nature

 LaCONESCSIR Takes Steps for Nurturing Nature

{Feature has been uploaded by CSIR (Unit for Science Dissemination), Ministry of Science & Technology, New Delhi}

What happens when our precious possessions are lost? Invariably, loss of wealth or something dear makes most of us quite upset that triggers us to take measures for protecting our belongings. Well, Nature too belongs to all of us. The bountiful Nature is replete with priceless possessions in the form of myriad varieties of life forms, each one of them being a masterpiece in itself. But alas, some of them like the Caspian tiger, Sea cow, Dodo, Quagga and many more are gone forever and we shall never see them again. With their extinction, the millions of years that these species took to evolve are also lost. Surely, loss of this natural wealth reflected by extinction of several plant and animal species from the face of the Earth is not only very disturbing but also is a fair reason for gearing up our action to conserve the endangered life forms that are on the verge of extinction.  
Needless to say, conservation of the existing biodiversity is the need of the hour. Several factors like destruction of forests and indiscriminate killing of animals as a sport and for trade has been the major cause for pushing animals to extinction.  It is a matter of pride that CSIR has taken a lead in conservation of wildlife as the Centre for Cellular and Molecular Biology (CCMB), Hyderabad, initiated in 1998 the setting up of the laboratory for Conservation of Endangered Species (LaCONES) with help of the Department of Biotechnology (DBT), Government of India, New Delhi and the Central Zoo Authority (CZA), New Delhi. The Department of Forestry, Government of Andhra Pradesh and the Nehru Zoological Park at Hyderabad, are also major partners in this joint venture.
Piloted by Dr. Lalji Singh, former Director, CCMB and Dr. S. Shivaji, Director-grade scientist, CCMB, LaCONES is a unique laboratory set up at Attapur near Nehru Zoological park, Hyderabad, on land allotted by the Government of Andhra Pradesh. This novel scientific endeavour is a part of a national programme to tackle the challenge of wildlife extinction by employing novel methods of biotechnology and assisted reproductive technologies. The idea is to conserve and prevent the extinction of endangered wildlife by focusing on both conservation as well as propagation of such species by several innovative techniques. It was on February 1, 2007 that the then President of India, Dr A. P. J. Abdul Kalam dedicated the LaCONES to the Nation for the cause of conservation of wildlife (CSIR News, Vol. 57, No. 4, 28 February 2007)
The wide range of research activities at the LaCONES includes the resurrection of extinct and endangered species by cloning the frozen genetic material and using oocytes of a closely related species as surrogate mother, monitoring of genetic variations and establishing of gene and cell banks for such species. A major focus is on the development of assisted reproduction technologies such as intrauterine insemination, in vitro fertilization, intra-cytoplasm sperm injection, embryo transfer, etc., for conservation of rare species.
The state-of-the-art LaCONES has facilities for cryo-preservation of semen, eggs and embryos of endangered species. Semen analysis is done to study the semen quality for selecting animals for breeding. Besides, standardization of artificial insemination for wild animals is another major activity at LaCONES. Planned in-house breeding strategies or captive breeding of animals that cannot be saved in the wild are developed. The outcome of such efforts has greatly helped in preventing extinction of wild animals like cheetah, non-human primates, and several birds among other rare species.
Protocols have been established for anaesthetizing and electro-ejaculating a wide range of animals such as lions, tigers, leopards, hyenas, jackals, bears, deers and monkeys. A mobile van equipped with facilities like cryopreservation, sonography, computerized sperm motility analyzer. etc., enables the scientists to reach different  habitats for collecting samples.
As DNA is the blueprint of life of all organisms, its long-term preservation is a crucial step in storing all information about various specifications of that life form. This has a futuristic possibility of reconstructing an extinct species, while providing a renewable resource for genetic variation in endangered species through captive breeding when loss of variation through inbreeding seriously threatens their survival. On the contrary, if the DNA and cells of endangered species were not stored, the vital information linked to these life forms would be lost forever. It is a heartening fact that the LaCONES has been honoured as a Member of the International Consortium of the Frozen Ark – an international depository of DNA representing the Indian subcontinent.
The Frozen Ark Consortium is a worldwide group of institutions, with its coordinating office located at the School of Biology, University of Nottingham, U.K. In the Frozen Ark freezers, the DNA and the viable cells of endangered species are stored in liquid nitrogen at -196oC. The CCMB scientists have developed primers to decode the molecular signatures of endangered species from their tissue samples by the technique of DNA fingerprinting. A database of molecular signatures of over 2000 animal species has been created.
Scientists at LaCONES have successfully employed the assisted reproductive technologies to propagate wildlife species such as black buck, chital and blue rock pigeon. The birth of ‘Spotty’, a baby spotted deer/cheetal, using artificial insemination in March 2006 was indeed breathtaking. Such a success story was repeated in August 2007 when ‘Blacky’, a black buck, was born to one of the three inseminated bucks. The same techniques are being employed on other rare species such as vultures, Nicobar pigeons and the big cats for increasing their dwindling numbers. The LaCONES is indeed a classic example of application of science & technology in protecting thousands of marvelous creations of Nature.


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